Flow Cytometry for the CCCP Cells:
- The first graph, titled All Events, has sorted cells according to their internal complexity and size.
- The red circle on this graph has isolated a population that are large and relatively complex, which are characteristic of luteal cells. This population is graphed on R1.
- R1 is gated to account for doublets. Doublets occur when two cells are analyzed by the flow cytometer at once, and are incorrectly sorted as a large complex cell.
- The remaining graphs are based on the population within the blue gate of R1.
- The graph titled YL1 measures fluorescence at 568 nanometers. The dye TMRM, which was used during this study, is excited at 488 nm; however, the graph is plotted in terms of 568 nm.
- In YL1, there was a decrease in the number of cells within the fluorescence perimeter: 45.175% were fluorescent opposed to the 88.973% in cells treated without CCCP